This course, which is held on the premises of the New York Structural Biology Center, will cover the theory and practice of solving molecular structures by electron microscopy. It blends a series of lectures from local experts followed by student-led discussion sessions with practical sessions that parallel the topics introduced during the lectures. The course first covers optics, sample preparation and a basic mathematical description of diffraction before moving into a detailed exploration of the three main methods of structure determination: tomography, single particle analysis and 2D crystallography. The course ends with a discussion of map interpretation and molecular fitting.
Elective for Year 3 and above, offered at the New York Structural Biology Center.