This elective course offers an overview of modern biophysical experimental techniques used in the study of biological systems at the cellular and molecular level. Topics include light microscopy, fluorescence microscopy, Fourier optics and image processing, confocal and multiphoton microscopy, evanescent-wave microscopy and fluorescence correlations spectroscopy, phase contrast, electron microscopy, x-ray diffraction, multidimensional NMR, chromophores, calcium measurements, resonance energy transfer, membrane biophysics, ion channels, action potentials, ligand-gated channels, fluctuation analysis, patch-clamping, rapid kinetics, caged compounds, transmitter release, capacitance measurements, amperometry, optical traps, molecular force measurements and computer modeling. While a basic knowledge of physics and mathematics is helpful, this is an interdisciplinary course designed for students with diverse backgrounds.