An overview of the diversity of modern biophysical experimental techniques used in the study of biological systems at the cellular and molecular level. Topics covered will include methods that examine both structure and function of biological systems. Topics include light microscopy, fluorescence microscopy, image processing, confocal and multiphoton microscopy, phase contrast, electron microscopy, x-ray diffraction and protein structure determination, multidimensional NMR, spectroscopy, chromophores, calcium measurements, resonance energy transfer, membrane biophysics, electrophysiology, ion channels, action potentials, ligand-gated channels, fluctuation analysis, patch-clamp, molecular biology of ion channels, capacitance measurements, amperometry, optical traps, and molecular force measurements. The course is intended for students who seek an introduction to modern biophysical experimental methods. Due to the interdisciplinary nature of the course, students will have diverse backgrounds. A basic knowledge of and interest in physics and mathematics is expected but strong attempts are made to give an intuitive understanding of the mathematics and physics involved. Some knowledge of physical chemistry, molecular and cell biology, or neurobiology will be helpful. Depending on individual background most students will find certain aspects easy and other aspects demanding.